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Original Research 
RMJ. 2022; 47(3): 540-543


Pattern of MICRO-RNA-196A2 Polymorphism among type 2 diabetics

Najia Soomro, Sabir Hussain, Ahsan Kazmi, Sarwat Batool, Syed Naqeeb Ali, Syed Liaquat Ali.

Abstract
Objective: To identify and determine the pattern of miRNA-196a2 (rs11614913) polymorphism among type 2 diabetics.
Methodology: This case control study was conducted on 311 individuals comprising 161 type 2 diabetics and 150 healthy controls. Genomic DNA was extracted by using non-enzymatic extraction method. Quality of extracted DNA was evaluated by horizontal gel electrophoresis on 1% agarose. The extracted samples were used to run PCR-RFLP for detecting polymorphism in miR-196a2 gene.
Results: Genotypes found were TT (normal genotype), TC (variant genotype) and CC (variant genotype). The miRNA variant (TC+CC) indicated a significant association with diabetes (69%) as compared to controls (53.3%) (χ 2= 7.98, P = 0.004).
Conclusion: The miRNA variants (TC+CC) indicated a significant association with type 2 diabetes as compared to controls. The percentage of miRNA-196a2 C allele was greater in patients as compared to controls while the frequency of T allele was less in patients compared to the controls.

Key words: Type 2 diabetes mellitus, miRNA-196a2, DNA.


 
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How to Cite this Article
Pubmed Style

Soomro N, Hussain S, Kazmi A, Batool S, Ali SN, Ali SL. Pattern of MICRO-RNA-196A2 Polymorphism among type 2 diabetics. RMJ. 2022; 47(3): 540-543.


Web Style

Soomro N, Hussain S, Kazmi A, Batool S, Ali SN, Ali SL. Pattern of MICRO-RNA-196A2 Polymorphism among type 2 diabetics. https://www.rmj.org.pk/?mno=27088 [Access: September 02, 2022].


AMA (American Medical Association) Style

Soomro N, Hussain S, Kazmi A, Batool S, Ali SN, Ali SL. Pattern of MICRO-RNA-196A2 Polymorphism among type 2 diabetics. RMJ. 2022; 47(3): 540-543.



Vancouver/ICMJE Style

Soomro N, Hussain S, Kazmi A, Batool S, Ali SN, Ali SL. Pattern of MICRO-RNA-196A2 Polymorphism among type 2 diabetics. RMJ. (2022), [cited September 02, 2022]; 47(3): 540-543.



Harvard Style

Soomro, N., Hussain, . S., Kazmi, . A., Batool, . S., Ali, . S. N. & Ali, . S. L. (2022) Pattern of MICRO-RNA-196A2 Polymorphism among type 2 diabetics. RMJ, 47 (3), 540-543.



Turabian Style

Soomro, Najia, Sabir Hussain, Ahsan Kazmi, Sarwat Batool, Syed Naqeeb Ali, and Syed Liaquat Ali. 2022. Pattern of MICRO-RNA-196A2 Polymorphism among type 2 diabetics. Rawal Medical Journal, 47 (3), 540-543.



Chicago Style

Soomro, Najia, Sabir Hussain, Ahsan Kazmi, Sarwat Batool, Syed Naqeeb Ali, and Syed Liaquat Ali. "Pattern of MICRO-RNA-196A2 Polymorphism among type 2 diabetics." Rawal Medical Journal 47 (2022), 540-543.



MLA (The Modern Language Association) Style

Soomro, Najia, Sabir Hussain, Ahsan Kazmi, Sarwat Batool, Syed Naqeeb Ali, and Syed Liaquat Ali. "Pattern of MICRO-RNA-196A2 Polymorphism among type 2 diabetics." Rawal Medical Journal 47.3 (2022), 540-543. Print.



APA (American Psychological Association) Style

Soomro, N., Hussain, . S., Kazmi, . A., Batool, . S., Ali, . S. N. & Ali, . S. L. (2022) Pattern of MICRO-RNA-196A2 Polymorphism among type 2 diabetics. Rawal Medical Journal, 47 (3), 540-543.








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