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Original Research 
RMJ. 2025; 50(2): 374-376


Diagnostics pathology insights to pro-apoptotic potential of eugenol against human cervical cancer cell line

Aafia Afridi, Maeesa Wadood, Abdul Rehman Khalil Shaikh, Abdul Mannan Jehangir, Madeeha Minhas, Haseeb Khaliq.


Abstract
Objective: To explore the anti-cancer potential of eugenol (4-allyl-2-methoxyphenol) on HeLa (Henrietta Lacks) human cervical cancer cell line.
Methodology: This In-vitro lab study worked on translating lab results into IC50 (half-maximal inhibitory concentration) values which depended on MTT assay-based outcomes [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. It is used for evaluating eugenol’s concentrations focused effects on HeLa cell lines while performing gene expression studies through RT-qPCR (Quantitative Reverse Transcription Polymerase Chain Reaction) analysis of apoptotic mediator genes (Intrinsic Biomarkers Caspases).
Results: Evaluation using IC50 revealed eugenol at 14.1 uM effectively induced pro-apoptotic biomarkers Caspase-3 and Caspase-9 in HeLa cells at relative gene expression levels of 12.9 and 12.3.
Conclusion: Our data confirmed robust pro-apoptotic anti-cancer properties of eugenol against HeLa cell lines. Future investigations need to discover the main pathways through which eugenol fights cancer in HeLa cell lines.

Key words: Eugenol, HeLa, eugenol, pro-apoptotic potential, anti-cancer activity.


 
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How to Cite this Article
Pubmed Style

Afridi A, Wadood M, Shaikh ARK, Jehangir AM, Minhas M, Khaliq H. Diagnostics pathology insights to pro-apoptotic potential of eugenol against human cervical cancer cell line. RMJ. 2025; 50(2): 374-376.

Web Style

Afridi A, Wadood M, Shaikh ARK, Jehangir AM, Minhas M, Khaliq H. Diagnostics pathology insights to pro-apoptotic potential of eugenol against human cervical cancer cell line. https://www.rmj.org.pk/?mno=240174 [Access: May 12, 2025].

AMA (American Medical Association) Style

Afridi A, Wadood M, Shaikh ARK, Jehangir AM, Minhas M, Khaliq H. Diagnostics pathology insights to pro-apoptotic potential of eugenol against human cervical cancer cell line. RMJ. 2025; 50(2): 374-376.


Vancouver/ICMJE Style

Afridi A, Wadood M, Shaikh ARK, Jehangir AM, Minhas M, Khaliq H. Diagnostics pathology insights to pro-apoptotic potential of eugenol against human cervical cancer cell line. RMJ. (2025), [cited May 12, 2025]; 50(2): 374-376.


Harvard Style

Afridi, A., Wadood, . M., Shaikh, . A. R. K., Jehangir, . A. M., Minhas, . M. & Khaliq, . H. (2025) Diagnostics pathology insights to pro-apoptotic potential of eugenol against human cervical cancer cell line. RMJ, 50 (2), 374-376.


Turabian Style

Afridi, Aafia, Maeesa Wadood, Abdul Rehman Khalil Shaikh, Abdul Mannan Jehangir, Madeeha Minhas, and Haseeb Khaliq. 2025. Diagnostics pathology insights to pro-apoptotic potential of eugenol against human cervical cancer cell line. Rawal Medical Journal, 50 (2), 374-376.


Chicago Style

Afridi, Aafia, Maeesa Wadood, Abdul Rehman Khalil Shaikh, Abdul Mannan Jehangir, Madeeha Minhas, and Haseeb Khaliq. "Diagnostics pathology insights to pro-apoptotic potential of eugenol against human cervical cancer cell line." Rawal Medical Journal 50 (2025), 374-376.


MLA (The Modern Language Association) Style

Afridi, Aafia, Maeesa Wadood, Abdul Rehman Khalil Shaikh, Abdul Mannan Jehangir, Madeeha Minhas, and Haseeb Khaliq. "Diagnostics pathology insights to pro-apoptotic potential of eugenol against human cervical cancer cell line." Rawal Medical Journal 50.2 (2025), 374-376. Print.


APA (American Psychological Association) Style

Afridi, A., Wadood, . M., Shaikh, . A. R. K., Jehangir, . A. M., Minhas, . M. & Khaliq, . H. (2025) Diagnostics pathology insights to pro-apoptotic potential of eugenol against human cervical cancer cell line. Rawal Medical Journal, 50 (2), 374-376.


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