Clinical manifestations, seroprevalence and evaluation of serological assays for Chikungunya Virus Infection

Shabnam Dildar; Saman Nadeem; Madiha Naqvi; Ashfaq Hussain; Maria Ali Ali; Shaista Bakhat

2025, Vol: 50, Issue: 4

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RMJ. 2025; 50(4): 851-853

Clinical manifestations, seroprevalence and evaluation of serological assays for Chikungunya Virus Infection

Shabnam Dildar, Saman Nadeem, Madiha Naqvi, Ashfaq Hussain, Maria Ali Ali, Shaista Bakhat.

Abstract
Objective: To explore the seroprevalence, clinical features, and effectiveness of serological tests for CHIKV infection at a tertiary care hospital in Karachi, Pakistan.
Methodology: This cross-sectional study was carried out from August to October 2024, involving patients with acute febrile illness and joint pain. Serological tests were conducted using ELISA and ELFA to detect anti-CHIKV IgM antibodies. The study utilized SPSS version 24 to analyze data across five age groups, with both the ELFA and ELISA assays assessed for their diagnostic accuracy in detecting anti-CHIKV IgM. Sensitivity, specificity, PPV, and NPV were calculated for both assays, and their agreement was evaluated.
Results: The overall seroprevalence of CHIKV infection was 13.5% via ELISA and 15.1% via ELFA. The most frequently reported clinical symptoms were fever and joint pain. Both ELISA and ELFA showed high sensitivity and specificity in detecting CHIKV infection.
Conclusion: The rise in seroprevalence during the monsoon and post-monsoon seasons indicates a pressing need for improved vector control strategies during these times. Additional research is necessary to examine the long-term effects of CHIKV infection and to formulate effective prevention and treatment approaches.

Key words: Aedes mosquitoes, Chikungunya virus (CHIKV), ELFA (Enzyme-Linked Fluorescent Assay), ELISA (Enzyme-Linked Immunosorbent Assay).

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Pubmed Style

Dildar S, Nadeem S, Naqvi M, Hussain A, Ali MA, Bakhat S. Clinical manifestations, seroprevalence and evaluation of serological assays for Chikungunya Virus Infection. RMJ. 2025; 50(4): 851-853.

Web Style

Dildar S, Nadeem S, Naqvi M, Hussain A, Ali MA, Bakhat S. Clinical manifestations, seroprevalence and evaluation of serological assays for Chikungunya Virus Infection. https://www.rmj.org.pk/?mno=238258 [Access: October 29, 2025].

AMA (American Medical Association) Style

Dildar S, Nadeem S, Naqvi M, Hussain A, Ali MA, Bakhat S. Clinical manifestations, seroprevalence and evaluation of serological assays for Chikungunya Virus Infection. RMJ. 2025; 50(4): 851-853.

Vancouver/ICMJE Style

Dildar S, Nadeem S, Naqvi M, Hussain A, Ali MA, Bakhat S. Clinical manifestations, seroprevalence and evaluation of serological assays for Chikungunya Virus Infection. RMJ. (2025), [cited October 29, 2025]; 50(4): 851-853.

Harvard Style

Dildar, S., Nadeem, . S., Naqvi, . M., Hussain, . A., Ali, . M. A. & Bakhat, . S. (2025) Clinical manifestations, seroprevalence and evaluation of serological assays for Chikungunya Virus Infection. RMJ, 50 (4), 851-853.

Turabian Style

Dildar, Shabnam, Saman Nadeem, Madiha Naqvi, Ashfaq Hussain, Maria Ali Ali, and Shaista Bakhat. 2025. Clinical manifestations, seroprevalence and evaluation of serological assays for Chikungunya Virus Infection. Rawal Medical Journal, 50 (4), 851-853.

Chicago Style

Dildar, Shabnam, Saman Nadeem, Madiha Naqvi, Ashfaq Hussain, Maria Ali Ali, and Shaista Bakhat. "Clinical manifestations, seroprevalence and evaluation of serological assays for Chikungunya Virus Infection." Rawal Medical Journal 50 (2025), 851-853.

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