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Original Research
Online Published: 24 Sep 2026
 
RMJ. 2026; 51(4): 966-970


Molecular study of drug resistance of Mycobacterium tuberculosis in Quetta, Pakistan

Mehrullah Kakar, Abrar Hussain, Shereen Khan, Pari Gul, Shumaiza Anis, Muhammad Asif.


Abstract
Objective: To determine the prevalence of Mycobacterium tuberculosis (MTB) across age groups and genders; compare diagnostic methods to identify the most appropriate method; and detect drug resistance and other related treatment complications using the LPA method.
Methodology: A total of 1500 TB-suspected individuals were enrolled. Fluorescent microscopy (FM) was performed at the time of diagnosis on all suspected individuals to confirm AFB (acid-fast bacilli) positivity and was stratified into four categories: 0+ (scanty), 1+ (low), 2+ (medium), and 3+ (high). To confirm the incidence of positive cases and rifamicin (RIF) resistance, GeneXpert (PCR) was performed on all suspects.
Results: Fluorescent microscopy showed that 5.07% was high in the (2+ medium) strata. At the time of diagnosis, PCR (GeneXpert) confirmed 130/1500 positive consistently, whereas in a total of 130 positive TB cases, 3 (2 males and 1 female) cases were those that showed resistance against rifampicin MTB. Further, to confirm RIF alone and a combination of (INH-R) resistance, the 3 resistant individuals were processed for LPA, in which 2 were resistant to just RIF, and 1 sample exhibited INH-R resistance.
Conclusion: Tuberculosis-positive cases were most prevalent in females and were relatively higher in the middle-aged group.

Key words: Fluorescence microscopy, GeneXpert, Line Probe Assay, drug resistance.


 
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How to Cite this Article
Pubmed Style

Kakar M, Hussain A, Khan S, Gul P, Anis S, Asif M. Molecular study of drug resistance of Mycobacterium tuberculosis in Quetta, Pakistan. RMJ. 2026; 51(4): 966-970.

Web Style

Kakar M, Hussain A, Khan S, Gul P, Anis S, Asif M. Molecular study of drug resistance of Mycobacterium tuberculosis in Quetta, Pakistan. https://www.rmj.org.pk/?mno=294789 [Access: November 11, 2026].

AMA (American Medical Association) Style

Kakar M, Hussain A, Khan S, Gul P, Anis S, Asif M. Molecular study of drug resistance of Mycobacterium tuberculosis in Quetta, Pakistan. RMJ. 2026; 51(4): 966-970.


Vancouver/ICMJE Style

Kakar M, Hussain A, Khan S, Gul P, Anis S, Asif M. Molecular study of drug resistance of Mycobacterium tuberculosis in Quetta, Pakistan. RMJ. (2026), [cited November 11, 2026]; 51(4): 966-970.


Harvard Style

Kakar, M., Hussain, . A., Khan, . S., Gul, . P., Anis, . S. & Asif, . M. (2026) Molecular study of drug resistance of Mycobacterium tuberculosis in Quetta, Pakistan. RMJ, 51 (4), 966-970.


Turabian Style

Kakar, Mehrullah, Abrar Hussain, Shereen Khan, Pari Gul, Shumaiza Anis, and Muhammad Asif. 2026. Molecular study of drug resistance of Mycobacterium tuberculosis in Quetta, Pakistan. Rawal Medical Journal, 51 (4), 966-970.


Chicago Style

Kakar, Mehrullah, Abrar Hussain, Shereen Khan, Pari Gul, Shumaiza Anis, and Muhammad Asif. "Molecular study of drug resistance of Mycobacterium tuberculosis in Quetta, Pakistan." Rawal Medical Journal 51 (2026), 966-970.


MLA (The Modern Language Association) Style

Kakar, Mehrullah, Abrar Hussain, Shereen Khan, Pari Gul, Shumaiza Anis, and Muhammad Asif. "Molecular study of drug resistance of Mycobacterium tuberculosis in Quetta, Pakistan." Rawal Medical Journal 51.4 (2026), 966-970. Print.


APA (American Psychological Association) Style

Kakar, M., Hussain, . A., Khan, . S., Gul, . P., Anis, . S. & Asif, . M. (2026) Molecular study of drug resistance of Mycobacterium tuberculosis in Quetta, Pakistan. Rawal Medical Journal, 51 (4), 966-970.



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