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Original Research 
RMJ. 2022; 47(3): 564-568


Bacteriological and antibiotic susceptibility profiling of the cerebrospinal fluid of suspected bacterial meningitis patients

Babar Ahmed Memon, Shaista Bano, Shah Muhammad Abbasi, Sarfraz Ali Tunio.

Abstract
Objective: To investigate the bacteriological and antibiotic susceptibility profile of cerebrospinal fluid specimens collected from the patients with suspected bacterial meningitis.
Methodology: This prospective, cross-sectional study was carried out in which the cerebrospinal fluid specimens were obtained by lumber puncture from the hospitalized patients with suspected bacterial meningitis from January to December 2019. The bacterial isolates were identified using microscopic observations and biochemical methods. Kirby-Bauer disc diffusion method was used for antibiotic susceptibility profiling of isolates.
Results: Out of 519 specimens, 105(20.23%) were positive, while 414(79.77%) were negative for any microbial growth. The most frequent isolates were Coagulase negative Staphylococcus spp (CoNS) (17.15%), Staphylococcus aureus (12.38%), Acinetobacter spp (17.14%), and Escherichia coli (9.52%), while species of Enterococcus, Streptococcus, Klebsiella, Pseudomonas, Proteus, Neisseria, and Haemophilus were also recovered. S. aureus isolates were resistant to penicillin (94.44%) and oxacillin (83.33%), while susceptible to linezolid, vancomycin, and amikacin. Similar observations were obtained from CoNS isolates. For E. coli, amikacin and meropenem remained effective with 80.0% and 60.0%, respectively. Importantly, 50% of Acinetobacter spp isolates were resistant to all the five antibiotics tested.
Conclusion: The pathogens causing bacterial meningitis appeared resistant to the commonly used antibiotics while linezolid remained the most effective for Gram-positive isolates. However, antibiotic resistance in Gram-negative isolates seems alarming and informs the need of antibiotic stewardship at Pakistan.

Key words: Bacterial meningitis, Staphylococcus aureus, Neisseria meningitidis, antibiotics.


 
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How to Cite this Article
Pubmed Style

Memon BA, Bano S, Abbasi SM, Tunio SA. Bacteriological and antibiotic susceptibility profiling of the cerebrospinal fluid of suspected bacterial meningitis patients. RMJ. 2022; 47(3): 564-568.


Web Style

Memon BA, Bano S, Abbasi SM, Tunio SA. Bacteriological and antibiotic susceptibility profiling of the cerebrospinal fluid of suspected bacterial meningitis patients. https://www.rmj.org.pk/?mno=88916 [Access: September 02, 2022].


AMA (American Medical Association) Style

Memon BA, Bano S, Abbasi SM, Tunio SA. Bacteriological and antibiotic susceptibility profiling of the cerebrospinal fluid of suspected bacterial meningitis patients. RMJ. 2022; 47(3): 564-568.



Vancouver/ICMJE Style

Memon BA, Bano S, Abbasi SM, Tunio SA. Bacteriological and antibiotic susceptibility profiling of the cerebrospinal fluid of suspected bacterial meningitis patients. RMJ. (2022), [cited September 02, 2022]; 47(3): 564-568.



Harvard Style

Memon, B. A., Bano, . S., Abbasi, . S. M. & Tunio, . S. A. (2022) Bacteriological and antibiotic susceptibility profiling of the cerebrospinal fluid of suspected bacterial meningitis patients. RMJ, 47 (3), 564-568.



Turabian Style

Memon, Babar Ahmed, Shaista Bano, Shah Muhammad Abbasi, and Sarfraz Ali Tunio. 2022. Bacteriological and antibiotic susceptibility profiling of the cerebrospinal fluid of suspected bacterial meningitis patients. Rawal Medical Journal, 47 (3), 564-568.



Chicago Style

Memon, Babar Ahmed, Shaista Bano, Shah Muhammad Abbasi, and Sarfraz Ali Tunio. "Bacteriological and antibiotic susceptibility profiling of the cerebrospinal fluid of suspected bacterial meningitis patients." Rawal Medical Journal 47 (2022), 564-568.



MLA (The Modern Language Association) Style

Memon, Babar Ahmed, Shaista Bano, Shah Muhammad Abbasi, and Sarfraz Ali Tunio. "Bacteriological and antibiotic susceptibility profiling of the cerebrospinal fluid of suspected bacterial meningitis patients." Rawal Medical Journal 47.3 (2022), 564-568. Print.



APA (American Psychological Association) Style

Memon, B. A., Bano, . S., Abbasi, . S. M. & Tunio, . S. A. (2022) Bacteriological and antibiotic susceptibility profiling of the cerebrospinal fluid of suspected bacterial meningitis patients. Rawal Medical Journal, 47 (3), 564-568.








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