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Original Research 
RMJ. 2023; 48(4): 1061-1064


Melatonin attenuates drug induced liver injury caused by combined effect of methotrexate and isoniazid

Aamna Khokhar, Sadia Shaukat, Zunera Hakim, Aisha Qayyum, Saima Rafique, Abeera Sikandar.


Abstract
Objective: To investigate the shielding effect of melatonin in drug induced liver injury (DILI) by isoniazid (INH) and methotrexate (MTX).
Methodology: This experimental study was performed at Department of Pharmacology, Army Medical College, Rawalpindi from November 2015 to November 2016. Thirty-six Balb/c male mice were split into 6 groups (n=6). Two groups (A1 and A2) served as controls for MTX and INH treated mice. Group B and C received MTX and INH, respectively. Group D received INH and MTX. Group E received INH and MTX along with melatonin. Serum liver enzymes and liver histological slides were taken after 24 hours of last dose.
Results: Serum liver enzymes were significantly elevated in group B (MTX) and in group D (MTX + INH) (p<0.001) but not significantly altered in group C (INH) as compared to its control group (p<0.560). Group D (MTX + INH) showed moderate to severe hepatotoxicity in histopathology. Group E (MTX + INH + Melatonin) displayed liver regenerative changes along with slight fall in ALT levels as compared to group D (MTX + INH).
Conclusion: Concomitant administration of melatonin was an effective strategy to prevent liver damage caused by MTX and INH in this rat model.

Key words: DILI, isoniazid, melatonin, methotrexate.


 
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How to Cite this Article
Pubmed Style

Khokhar A, Shaukat S, Hakim Z, Qayyum A, Rafique S, Sikandar A. Melatonin attenuates drug induced liver injury caused by combined effect of methotrexate and isoniazid. RMJ. 2023; 48(4): 1061-1064.

Web Style

Khokhar A, Shaukat S, Hakim Z, Qayyum A, Rafique S, Sikandar A. Melatonin attenuates drug induced liver injury caused by combined effect of methotrexate and isoniazid. https://www.rmj.org.pk/?mno=85097 [Access: October 30, 2025].

AMA (American Medical Association) Style

Khokhar A, Shaukat S, Hakim Z, Qayyum A, Rafique S, Sikandar A. Melatonin attenuates drug induced liver injury caused by combined effect of methotrexate and isoniazid. RMJ. 2023; 48(4): 1061-1064.


Vancouver/ICMJE Style

Khokhar A, Shaukat S, Hakim Z, Qayyum A, Rafique S, Sikandar A. Melatonin attenuates drug induced liver injury caused by combined effect of methotrexate and isoniazid. RMJ. (2023), [cited October 30, 2025]; 48(4): 1061-1064.


Harvard Style

Khokhar, A., Shaukat, . S., Hakim, . Z., Qayyum, . A., Rafique, . S. & Sikandar, . A. (2023) Melatonin attenuates drug induced liver injury caused by combined effect of methotrexate and isoniazid. RMJ, 48 (4), 1061-1064.


Turabian Style

Khokhar, Aamna, Sadia Shaukat, Zunera Hakim, Aisha Qayyum, Saima Rafique, and Abeera Sikandar. 2023. Melatonin attenuates drug induced liver injury caused by combined effect of methotrexate and isoniazid. Rawal Medical Journal, 48 (4), 1061-1064.


Chicago Style

Khokhar, Aamna, Sadia Shaukat, Zunera Hakim, Aisha Qayyum, Saima Rafique, and Abeera Sikandar. "Melatonin attenuates drug induced liver injury caused by combined effect of methotrexate and isoniazid." Rawal Medical Journal 48 (2023), 1061-1064.


MLA (The Modern Language Association) Style

Khokhar, Aamna, Sadia Shaukat, Zunera Hakim, Aisha Qayyum, Saima Rafique, and Abeera Sikandar. "Melatonin attenuates drug induced liver injury caused by combined effect of methotrexate and isoniazid." Rawal Medical Journal 48.4 (2023), 1061-1064. Print.


APA (American Psychological Association) Style

Khokhar, A., Shaukat, . S., Hakim, . Z., Qayyum, . A., Rafique, . S. & Sikandar, . A. (2023) Melatonin attenuates drug induced liver injury caused by combined effect of methotrexate and isoniazid. Rawal Medical Journal, 48 (4), 1061-1064.


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