ISSN 0303-5212
 

Original Research 
RMJ. 2024; 49(3): 661-664


In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line

Hafiz Muhammad Haseeb Khaliq, Owais Aziz, Monika Wadhwa, Shahla Imran.


Abstract
Objective: To investigate the pro-apoptotic potential of capsaicin on Human Leukemia HL-60 cell line as a potent natural chemotherapeutic agent.
Methodology: This study was conducted at Forman Christian College, Lahore from January to April-2024. It included culturing HL-60 cell line and its treatment with serial concentrations of capsaicin for calculation of subsequent IC50 values via MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay, expression analysis of gene, comparative analysis of relative gene fold (Intrinsic Biomarkers Caspase-3, Caspase-9) as apoptosis mediator markers followed by RT-qPCR. Cellular apoptotic morphology was confirmed via Hoechst 333258 staining.
Results: For HL-60 cell line, the IC50 of capsaicin (16.7uM) showed high gene expression of pro-apoptotic biomarkers (Caspase-3 and Caspase-9). Hoechst 333258 staining showed prominent apoptotic bodies leading to nuclear fragmentations.
Conclusion: Capsaicin had robust pro-apoptotic potential leading to diagnostic efficacy against leukemia HL-60 cell line. Further studies would help in identifying key mechanisms by which capsaicin exhibits anti-apoptotic potential against HL-60 cell lines.

Key words: Capsaicin, pro-apoptotic effects, anti-cancer mechanisms, HL-60, natural chemotherapeutics.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Hafiz Muhammad Haseeb Khaliq
Articles by Owais Aziz
Articles by Monika Wadhwa
Articles by Shahla Imran
on Google
on Google Scholar

How to Cite this Article
Pubmed Style

Khaliq HMH, Aziz O, Wadhwa M, Imran S. In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. RMJ. 2024; 49(3): 661-664.


Web Style

Khaliq HMH, Aziz O, Wadhwa M, Imran S. In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. https://www.rmj.org.pk/?mno=201867 [Access: November 17, 2024].


AMA (American Medical Association) Style

Khaliq HMH, Aziz O, Wadhwa M, Imran S. In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. RMJ. 2024; 49(3): 661-664.



Vancouver/ICMJE Style

Khaliq HMH, Aziz O, Wadhwa M, Imran S. In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. RMJ. (2024), [cited November 17, 2024]; 49(3): 661-664.



Harvard Style

Khaliq, H. M. H., Aziz, . O., Wadhwa, . M. & Imran, . S. (2024) In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. RMJ, 49 (3), 661-664.



Turabian Style

Khaliq, Hafiz Muhammad Haseeb, Owais Aziz, Monika Wadhwa, and Shahla Imran. 2024. In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. Rawal Medical Journal, 49 (3), 661-664.



Chicago Style

Khaliq, Hafiz Muhammad Haseeb, Owais Aziz, Monika Wadhwa, and Shahla Imran. "In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line." Rawal Medical Journal 49 (2024), 661-664.



MLA (The Modern Language Association) Style

Khaliq, Hafiz Muhammad Haseeb, Owais Aziz, Monika Wadhwa, and Shahla Imran. "In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line." Rawal Medical Journal 49.3 (2024), 661-664. Print.



APA (American Psychological Association) Style

Khaliq, H. M. H., Aziz, . O., Wadhwa, . M. & Imran, . S. (2024) In vitro insights: Investigating pro-apoptotic potential of capsaicin against HL-60, Human Leukemia cell line. Rawal Medical Journal, 49 (3), 661-664.