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2023, Vol: 48, Issue: 2
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Original Research 
RMJ. 2023; 48(3): 635-638


Detection of human papillomavirus genotypes (HPV-16) and (HPV-18) in women with abnormal cervical cytology in Al-Diwaniyah Province, Iraq

Khawlah Jabbar Hussein, Anwar Salih Saihood, Yasamin Hamza Sharif.

Abstract
Objective: To identify the human papillomavirus (HPV) genotypes; HPV-16 and HPV-18 from women with abnormal cervical cytology.
Methodology: In this cross-sectional study, 100 females 17 to 60 years of age (mean: 34 years) with abnormal cervical cytology were included. For each woman, two tissue specimens i.e. one for a pap smear and one for a genetic study were collected. The genetic study was carried out by using conventional polymerase chain reaction (PCR) method for confirmation of diagnosis. A real-time PCR (RT-PCR) was done on positive samples for the detection of the genotypes.
Results: We found that 64 (64%) out of 100 patients had abnormal tissue findings. The RT-PCR demonstrated that 32 (88.88%) samples were positive for the HPV 16 and HPV 18, at 20 cycles (55.55%) and 12 cycles (33.33%), respectively, with four (11.11%) samples showing unknown genotypic identity.
Conclusion: The present investigation provides novel information about the status of the human papillomavirus infection in women from Al-Diwaniyah Province with important reporting of HPV-16 and HPV-18 genotypes. Samples of this Province are mainly positive for the HPV 16 and HPV 18 with high percentages.

Key words: Cervix cancer, Human papillomavirus, Pap smear, patient.


 
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How to Cite this Article
Pubmed Style

Hussein KJ, ASS, Sharif YH. Detection of human papillomavirus genotypes (HPV-16) and (HPV-18) in women with abnormal cervical cytology in Al-Diwaniyah Province, Iraq. RMJ. 2023; 48(3): 635-638.

Web Style

Hussein KJ, ASS, Sharif YH. Detection of human papillomavirus genotypes (HPV-16) and (HPV-18) in women with abnormal cervical cytology in Al-Diwaniyah Province, Iraq. https://www.rmj.org.pk/?mno=150485 [Access: September 03, 2023].

AMA (American Medical Association) Style

Hussein KJ, ASS, Sharif YH. Detection of human papillomavirus genotypes (HPV-16) and (HPV-18) in women with abnormal cervical cytology in Al-Diwaniyah Province, Iraq. RMJ. 2023; 48(3): 635-638.


Vancouver/ICMJE Style

Hussein KJ, ASS, Sharif YH. Detection of human papillomavirus genotypes (HPV-16) and (HPV-18) in women with abnormal cervical cytology in Al-Diwaniyah Province, Iraq. RMJ. (2023), [cited September 03, 2023]; 48(3): 635-638.


Harvard Style

Hussein, K. J., , . A. S. S. & Sharif, . Y. H. (2023) Detection of human papillomavirus genotypes (HPV-16) and (HPV-18) in women with abnormal cervical cytology in Al-Diwaniyah Province, Iraq. RMJ, 48 (3), 635-638.


Turabian Style

Hussein, Khawlah Jabbar, Anwar Salih Saihood, and Yasamin Hamza Sharif. 2023. Detection of human papillomavirus genotypes (HPV-16) and (HPV-18) in women with abnormal cervical cytology in Al-Diwaniyah Province, Iraq. Rawal Medical Journal, 48 (3), 635-638.


Chicago Style

Hussein, Khawlah Jabbar, Anwar Salih Saihood, and Yasamin Hamza Sharif. "Detection of human papillomavirus genotypes (HPV-16) and (HPV-18) in women with abnormal cervical cytology in Al-Diwaniyah Province, Iraq." Rawal Medical Journal 48 (2023), 635-638.


MLA (The Modern Language Association) Style

Hussein, Khawlah Jabbar, Anwar Salih Saihood, and Yasamin Hamza Sharif. "Detection of human papillomavirus genotypes (HPV-16) and (HPV-18) in women with abnormal cervical cytology in Al-Diwaniyah Province, Iraq." Rawal Medical Journal 48.3 (2023), 635-638. Print.


APA (American Psychological Association) Style

Hussein, K. J., , . A. S. S. & Sharif, . Y. H. (2023) Detection of human papillomavirus genotypes (HPV-16) and (HPV-18) in women with abnormal cervical cytology in Al-Diwaniyah Province, Iraq. Rawal Medical Journal, 48 (3), 635-638.


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