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Original Research 


Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq

Shymaa A.G Al hussainy, Lamees A Abdul-Lateef.

Abstract
Objectives: To use RAPD analysis to rapidly detects genomic polymorphisms.
Methodology: Diagnosis and genotyping of E. faecalis by using specific primer.
Results: out of the 105 specimens, 70 (66.6%) had a positive bacterial culture. The remaining 35 (33.3%) showed no growth. Out 70 positive samples, 15 (14.2%) cultured on chromogenic ager medium were found to be E. faecalis. Following the molecular detection method use of a specific primer based on the D-alanine D-alanine ligase gene as a genetic marker for E. faecalis by PCR, the results showed that all isolates were positive for ddlE. RAPD-PCR was used to determine the relationships between 15 E. faecalis isolates. M13 primer shown polymorphism in the isolates tested, yielding 7-14 bands with sizes ranging from 95 to 3000 bp. Two clusters formed the cladogram, which divided the 15 isolates. The first cluster (A) included one isolate, whereas the second cluster (B) contained 14 isolates.
Conclusion: The RAPD analysis indicated a clonal dissemination among most of the selected enterococcal isolates, which suggested they belonged to the same isolate

Key words: E. faecalis, ddlE, RAPD-PCR.


 
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Pubmed Style

hussainy SAA, Abdul-Lateef LA. Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq. RMJ. 2023; 48(4): 822-826. doi:10.5455/rmj.20230309083906

Web Style

hussainy SAA, Abdul-Lateef LA. Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq. https://www.rmj.org.pk/?mno=145860 [Access: December 05, 2023]. doi:10.5455/rmj.20230309083906

AMA (American Medical Association) Style

hussainy SAA, Abdul-Lateef LA. Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq. RMJ. 2023; 48(4): 822-826. doi:10.5455/rmj.20230309083906


Vancouver/ICMJE Style

hussainy SAA, Abdul-Lateef LA. Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq. RMJ. (2023), [cited December 05, 2023]; 48(4): 822-826. doi:10.5455/rmj.20230309083906


Harvard Style

hussainy, S. A. A. & Abdul-Lateef, . L. A. (2023) Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq. RMJ, 48 (4), 822-826. doi:10.5455/rmj.20230309083906


Turabian Style

hussainy, Shymaa A.G Al, and Lamees A Abdul-Lateef. 2023. Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq. Rawal Medical Journal, 48 (4), 822-826. doi:10.5455/rmj.20230309083906


Chicago Style

hussainy, Shymaa A.G Al, and Lamees A Abdul-Lateef. "Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq." Rawal Medical Journal 48 (2023), 822-826. doi:10.5455/rmj.20230309083906


MLA (The Modern Language Association) Style

hussainy, Shymaa A.G Al, and Lamees A Abdul-Lateef. "Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq." Rawal Medical Journal 48.4 (2023), 822-826. Print. doi:10.5455/rmj.20230309083906


APA (American Psychological Association) Style

hussainy, S. A. A. & Abdul-Lateef, . L. A. (2023) Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq. Rawal Medical Journal, 48 (4), 822-826. doi:10.5455/rmj.20230309083906


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