ISSN 0303-5212
 

Original Research 
RMJ. 2017; 42(3): 396-399


ILED fluorescent microscope in laboratory diagnosis of tuberculosis

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel.

Abstract
Objective: To assess new fluorescent technique in comparison with conventional ZN technique to improve detection of mycobacterium tuberculosis (TB) in Sudan.
Methodology: This case control study was conducted at Khartoum state Hospitals during December 2010. Three sputum samples were collected on 2 consecutive days from each one of 80 patient and was stained by ZN stain and fluorescent stain.
Results: From 80 patients suspected of tuberculosis, proportion of positive smears that detected were 17% and 33% for the ZN and auramine – O staining methods. The mean time of ZN technique in reading the result was 101 sec compared with fluorescent technique in reading of the result was 33.1 sec.
Conclusion: Fluorescent stain is more sensitive by 16% than ZN stain for diagnosis of TB. Fluorescent stain is three times rapid in detection more than ZN stain. Using this method to diagnose TB will be easy and less time consuming and will give accurate result.

Key words: ILED fluorescent microscope, laboratory analysis, tuberculosis.


 
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How to Cite this Article
Pubmed Style

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel. ILED fluorescent microscope in laboratory diagnosis of tuberculosis. RMJ. 2017; 42(3): 396-399.


Web Style

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel. ILED fluorescent microscope in laboratory diagnosis of tuberculosis. https://www.rmj.org.pk/?mno=257134 [Access: December 05, 2023].


AMA (American Medical Association) Style

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel. ILED fluorescent microscope in laboratory diagnosis of tuberculosis. RMJ. 2017; 42(3): 396-399.



Vancouver/ICMJE Style

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel. ILED fluorescent microscope in laboratory diagnosis of tuberculosis. RMJ. (2017), [cited December 05, 2023]; 42(3): 396-399.



Harvard Style

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel (2017) ILED fluorescent microscope in laboratory diagnosis of tuberculosis. RMJ, 42 (3), 396-399.



Turabian Style

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel. 2017. ILED fluorescent microscope in laboratory diagnosis of tuberculosis. Rawal Medical Journal, 42 (3), 396-399.



Chicago Style

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel. "ILED fluorescent microscope in laboratory diagnosis of tuberculosis." Rawal Medical Journal 42 (2017), 396-399.



MLA (The Modern Language Association) Style

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel. "ILED fluorescent microscope in laboratory diagnosis of tuberculosis." Rawal Medical Journal 42.3 (2017), 396-399. Print.



APA (American Psychological Association) Style

Amin Omer Ibrahim Abbas, Entisar Elhaj Elrayah, Fathelrahman Mahd Hassan Gameel (2017) ILED fluorescent microscope in laboratory diagnosis of tuberculosis. Rawal Medical Journal, 42 (3), 396-399.