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Original Research 
RMJ. 2020; 45(2): 350-352


Detection of biofilm and bacterial colonization on DJ stent in obstructive uropathy

Ghulam Shabir Shaikh, Abdul Hussain Shar, Muhammad Imran Soomro, Aamir Ali Shaikh, Nisar Ahmed Shaikh, Malik Hussain Jalbani.

Abstract
Objective: To detect biofilm development and to see the culture and sensitivity on DJ stent in obstructive uropathy.
Methodology: This descriptive cross sectional study was conducted at Urology, Pathology and Microbiology Department of Chandka Medical College Hospital SMBBMU Larkana and SALU Khairpur from January 1, to July 31, 2018. DJ stents were removed from all patients either General or in Local Anesthesia with the help of cystoscope and foreign body forceps. Lower part of stent was cut for biofilm. Lower part of DJ stent was cleared with normal saline to eradicate surface contaminants then shifted to tryptic soy broth to see culture and sensitivity.
Results: Out of 55 patients, 35 were male and 20 females. Mean age of males was 2713.1 and female 2213.8 years. Among them, 15(27.27%) were found to form biofilm, which specially occurred in those patient who had DJ for longer than six weeks. Biofilm formation was seen in 10 out of 35 (28.57%) males 5 out of 20 (25%) females. Stent culture was positive in 22 out of 55 (40%) patients. It was positive in 14 out of 35 (40%) in males 8 out of 20 (40%) females. The most common organisms seen were Escherichia coli in 15 (68.18%), Pseudomonas in 2(13.3%), Klebsiella in 2(13.3%), Enterococci in 1(6.6%) and Staphylococcus coagulase negative in 2(13.3%) patients.
Conclusion: Biofilm formation and positive cultures are common in any period of time, so removal of DJ stent should be well-planned with appropriate antibiotic prophylaxis to avoid complications like systematic inflammatory response syndrome and urosepsis.

Key words: DJ stent, Biofilm development, Culture and sensitivity


 
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How to Cite this Article
Pubmed Style

Shaikh GS, Shar AH, Soomro MI, Shaikh AA, Shaikh NA, Jalbani MH. Detection of biofilm and bacterial colonization on DJ stent in obstructive uropathy. RMJ. 2020; 45(2): 350-352.


Web Style

Shaikh GS, Shar AH, Soomro MI, Shaikh AA, Shaikh NA, Jalbani MH. Detection of biofilm and bacterial colonization on DJ stent in obstructive uropathy. http://www.rmj.org.pk/?mno=58776 [Access: May 10, 2020].


AMA (American Medical Association) Style

Shaikh GS, Shar AH, Soomro MI, Shaikh AA, Shaikh NA, Jalbani MH. Detection of biofilm and bacterial colonization on DJ stent in obstructive uropathy. RMJ. 2020; 45(2): 350-352.



Vancouver/ICMJE Style

Shaikh GS, Shar AH, Soomro MI, Shaikh AA, Shaikh NA, Jalbani MH. Detection of biofilm and bacterial colonization on DJ stent in obstructive uropathy. RMJ. (2020), [cited May 10, 2020]; 45(2): 350-352.



Harvard Style

Shaikh, G. S., Shar, . A. H., Soomro, . M. I., Shaikh, . A. A., Shaikh, . N. A. & Jalbani, . M. H. (2020) Detection of biofilm and bacterial colonization on DJ stent in obstructive uropathy. RMJ, 45 (2), 350-352.



Turabian Style

Shaikh, Ghulam Shabir, Abdul Hussain Shar, Muhammad Imran Soomro, Aamir Ali Shaikh, Nisar Ahmed Shaikh, and Malik Hussain Jalbani. 2020. Detection of biofilm and bacterial colonization on DJ stent in obstructive uropathy. Rawal Medical Journal, 45 (2), 350-352.



Chicago Style

Shaikh, Ghulam Shabir, Abdul Hussain Shar, Muhammad Imran Soomro, Aamir Ali Shaikh, Nisar Ahmed Shaikh, and Malik Hussain Jalbani. "Detection of biofilm and bacterial colonization on DJ stent in obstructive uropathy." Rawal Medical Journal 45 (2020), 350-352.



MLA (The Modern Language Association) Style

Shaikh, Ghulam Shabir, Abdul Hussain Shar, Muhammad Imran Soomro, Aamir Ali Shaikh, Nisar Ahmed Shaikh, and Malik Hussain Jalbani. "Detection of biofilm and bacterial colonization on DJ stent in obstructive uropathy." Rawal Medical Journal 45.2 (2020), 350-352. Print.



APA (American Psychological Association) Style

Shaikh, G. S., Shar, . A. H., Soomro, . M. I., Shaikh, . A. A., Shaikh, . N. A. & Jalbani, . M. H. (2020) Detection of biofilm and bacterial colonization on DJ stent in obstructive uropathy. Rawal Medical Journal, 45 (2), 350-352.








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