Open Access and Peer-reviewed
Home Journal Issues Guide for Authors Editorial Board Aims & Scope About Journal News & Announcements


Original Research 
RMJ. 2019; 44(3): 477-481


Immunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan

Abdul Aziz Shaikh aziz, Shaista Khan, Saira Bugti, Junaid Ali Thebo, Ghulam Nabi Rajper, Muhammad Hassan Khaskheli.

Abstract
Objective: To confirm Hematoxylin and Eosin (H&E) diagnosed and suspicious cases of Hodgkin’s lymphoma then sub-classify & differentiate from other mimicries based on panel of CD markers.
Methodology: This descriptive cross sectional study used non-probability purposive sampling and cases were collected at Department of Pathology, Liaquat University of Medical and Health Sciences (LUMHS) Jamshoroa and Diagnostic and Research Laboratory Hyderabad. Chi square test was used to compare discrete variables. Pearson’s correlation coefficients were used to test the correlation between continuous variables.
Results: Out of 100 cases, 80 were of Hodgkin's lymphoma (HL), based on panel of Cluster differentiation (CD) markers expression. Among these, 70 were of Classical Hodgkin's lymphoma (CHL), based on either positivity of both CD30 and CD15 or any one of them with negativity of CD45 for H&RS cells. The remainders 10 were of Nodular lymphocyte predominant Hodgkin's lymphoma (NLPHL), showing positivity for CD45 and negativity for CD30 & CD15 for Lymphocytic & Histiocytic (L&H) cells in all cases, but also shown positivity for CD20 in 90% cases.
Conclusion: The Hodgkin’s lymphoma originates from mature germinal center B-lymphocyte with phenotypically expression of CD 15 & CD 30, but in our investigation of immunochemistry showed that CD 20 positivity is not unusual. Lymphocytic and histiocytic cells of NLPHL expressed CD20 in 90% and CD45 in 100% cases This is mandatory for H&E diagnosed and suspicious cases of HL, to confirm on panel of CD markers, before giving the opinion.

Key words: Hodgkin's disease, Non Hodgkin lymphoma, CD markers, Nodular lymphocyte.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Abdul Aziz Shaikh aziz
Articles by Shaista Khan
Articles by Saira Bugti
Articles by Junaid Ali Thebo
Articles by Ghulam Nabi Rajper
Articles by Muhammad Hassan Khaskheli
on Google
on Google Scholar
Article Statistics
 Viewed: 821
Downloaded: 2
Cited: 0


How to Cite this Article
Pubmed Style

aziz AAS, Khan S, Bugti S, Thebo JA, Rajper GN, Khaskheli MH. Immunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan. RMJ. 2019; 44(3): 477-481.


Web Style

aziz AAS, Khan S, Bugti S, Thebo JA, Rajper GN, Khaskheli MH. Immunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan. http://www.rmj.org.pk/?mno=10964 [Access: August 23, 2019].


AMA (American Medical Association) Style

aziz AAS, Khan S, Bugti S, Thebo JA, Rajper GN, Khaskheli MH. Immunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan. RMJ. 2019; 44(3): 477-481.



Vancouver/ICMJE Style

aziz AAS, Khan S, Bugti S, Thebo JA, Rajper GN, Khaskheli MH. Immunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan. RMJ. (2019), [cited August 23, 2019]; 44(3): 477-481.



Harvard Style

aziz, A. A. S., Khan, . S., Bugti, . S., Thebo, . J. A., Rajper, . G. N. & Khaskheli, . M. H. (2019) Immunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan. RMJ, 44 (3), 477-481.



Turabian Style

aziz, Abdul Aziz Shaikh, Shaista Khan, Saira Bugti, Junaid Ali Thebo, Ghulam Nabi Rajper, and Muhammad Hassan Khaskheli. 2019. Immunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan. Rawal Medical Journal, 44 (3), 477-481.



Chicago Style

aziz, Abdul Aziz Shaikh, Shaista Khan, Saira Bugti, Junaid Ali Thebo, Ghulam Nabi Rajper, and Muhammad Hassan Khaskheli. "Immunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan." Rawal Medical Journal 44 (2019), 477-481.



MLA (The Modern Language Association) Style

aziz, Abdul Aziz Shaikh, Shaista Khan, Saira Bugti, Junaid Ali Thebo, Ghulam Nabi Rajper, and Muhammad Hassan Khaskheli. "Immunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan." Rawal Medical Journal 44.3 (2019), 477-481. Print.



APA (American Psychological Association) Style

aziz, A. A. S., Khan, . S., Bugti, . S., Thebo, . J. A., Rajper, . G. N. & Khaskheli, . M. H. (2019) Immunohistochemical analysis of Hodgkin’s lymphoma in population of Sindh, Pakistan. Rawal Medical Journal, 44 (3), 477-481.








AUTHOR LOGIN

REVIEWER LOGIN

Indexed
&
Abstracted


Indexed in WHO Index Medicus IMEMR, Emromedex, Pakmedinet, ExtraMED and Scopus

  Approved by the Higher Education Commission of Pakistan and Pakistan Medical and Dental Council


ABOUT JOURNAL
POLICIES
STATEMENTS

This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the Budapest Open Access Initiative (BOAI) definition of open access.

The articles in Rawal Medical Journal are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.